Physiomer® reduces the chemokine interleukin-;8 production by activated human respiratory epithelial cells

Physiomer e IL8 (PDF).

The authors have recently shown that the transcription factor nuclear factor-B (NF-B) is a central mediator in the NaCl-mediated interleukin (IL)-;8 production by human airway epithelial cells. In this study, it was investigated whether Physiomer®, an isotonic sea water-derived solution commercialized for cleaning the nasal mucosa, impaired the chemokine IL-;8 expression and secretion by human respiratory epithelial cells compared with that obtained with an isotonic 9% NaCl solution.
Primary human bronchial gland (HBG) epithelial cells were incubated either in Physiomer® or in a NaCl 9% solution and activated either with 20 ng·mL–1 tumour necrosis factor-;, or IL-;1ß, respectively. Physiomer® significantly reduced the IL-;8 protein release in basal and activated HBGcells in comparison with that obtained with the 9% NaCl solution. In contrast to the effects of Physiomer® observed on restingHBG cells, Physiomer® did not significantly reduce the level of phosphorylation of the NF-B inhibitor protein IB or the steady-state IL-;8 messenger ribonucleic acid levels in activated HBG cells, suggesting that Physiomer® would have a post-transcriptional effect on IL-;8 expression in activated HBG cells. The authors conclude that Physiomer® is potentially useful in the reduction of airway mucosal inflammation.