Effects of Desalted Deep Seawater on Hematologic and Blood Chemical Values in Mice

Variables hematológicas en ratones (pdf) 

Various processed foods and beverages have been manufactured using deep seawater (DSW), desalted DSW (dDSW), and concentrated DSW in Japan. To confirm the safety of dDSW, we investigated hematologic and blood chemical effects of dDSW in mice. The dDSW and desalted surface seawater (dSSW) were diluted to 6.7%, 10%, and 20% with purified water. BALB/c mice were housed for 12 weeks, and administered the diluted dDSW, dSSW, or purified water as a control during the period. The results for dDSW were compared with those for dSSW and purified water. None of the groups of mice showed any clear abnormal growth or behavior; neither did any show signs of illness nor a single case of death during the 12 weeks study. We found no significant differences between the dDSW and control groups in terms of red blood cell count, hemoglobin, hematocrit, white blood cell count, and neutrophil counts, whereas white blood cell and lymphocyte counts were significantly higher in the 10% dSSW group at the end of 4 and 12 weeks than those in the control group. A significantly higher triglyceride level was detected only in the 6.7% dSSW group. Our results show no evidence of acute or subacute effects of diluted dDSW. Effects of diluted dDSW on hematologic and blood chemical values in mice are thought to be similar to those of purified water. This finding suggests that dDSW is as safe as purified water for drinking water.

The application of deep sea water in Japan

Usos de agua de mar profunda en Japón (pdf)

Deep sea water (from a depth of more than 200 m) has cold temperature, abundant nutrients, and good water quality that is pathogen-free and stable. Basic research on the utilization of this water for fisheries in Japan began in 1976 and at present, deep-seawater pumping systems are established in Toyama and Kochi Prefectures and under construction in Shizuoka and Okinawa Prefectures. The research emphasis of many national organizations, prefectures, universities, and private companies is shifting from basic research to feasibility studies or practical applications of deep sea water. For example, in Kochi Prefecture, located in southern Japan, it was found that deep sea water is advantageous in the aquaculture of cold-water species. Current fisheries-related projects include:

• aquaculture (sea vegetables, fishes, shellfish, etc.)
• basic research on deep sea organisms
• restoration of sea grass habitats

A wide range of projects unrelated to fisheries that are utilizing deep sea water to develop new industries and contribute to local economies include:

• the food industry
• medical treatment facilities
• cooling water for power stations
• agriculture of cold climate vegetables

Future investigations should focus on further explorations of deep sea water attributes, a cascade system for using
deep sea water, reduction of costs, and potential environmental impacts.

Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic Eczema/dermatitis syndrome (AEDS)

Toma agua de mar Japón-artículo (pdf)

Deep sea water intake improves skin symptoms and mineral imbalance and decreases serum IgE levels mad IgEinducing cytokines, IL-4, IL-13 and IL-18 in patients with atopic eczema/dermatitis syndrome (AEDS), while distilled water intake fails to do so.

Magnesium (Mg) is involved in the regulation of immune responses. It has been reported that in Mg deficient rats, serum levels of substance P, histamine, interleukin-1 (IL-1), IL-6 and tumor necrosis factor-alpha were elevated. Mg deficiency also caused atopic eczema/dermatitis syndrome (AEDS)-like symptoms in hairless rats.
We have previously reported that drinking of refined deep sea water that contains Mg as its main cation signigicantly reduces whole blood flow time and blood pressure in healthy volunteers. We have now studied the effect of drinking deep sea water on patients with AEDS.

An Evaluation of Factors Affecting the Survival of Escherichia coli in Sea Water

Sobrevivencia de E Coli en AM (PDF).

D’Hérelle (1926) suggested that bacteriophages contribute to the self-purification process in natural waters, but ZoBell (1946) reported that they occurred sporadically and only in the littoral zone and concluded that there was insufficient evidence for bacteriophages to be considered of importance in limiting the bacterial population of the open ocean. Nevertheless, it was repeatedly stated in the literature (Carlucci and Pramer, 1959) that bacteriophages contribute to the rapid death and paucity of bacteria in sea water. Recent studies (Kriss and Rukina, 1947; Spencer, 1955) have shown that bacteriophages are not limited to the littoral zone but occur at points distant from land and at depths as great as 2000 meters. The bacteriophage isolated (1955) and studied (1957) by Spencer was active against several strains of the luminous marine bacterium Photobacterium phosphoreum. It caused lysis of host cells on sea water agar but not on tap water agar and appeared to be indigenous to the sea.
The present report describes the occurrence, persistence, and activity of some bacteriophages in sea water.

Management of post-ethmoidectomy crust formation: Randomized single-blind clinical trial comparing pressurized seawater versus antiseptic/mucolytic saline

Rhinology-AM nasal (PDF).

This study compared the efficacy of mechanical nasal lavages with pressurized seawater versus nasal irrigations with saline plus benzododecinium (antiseptic) plus oleosorbate (mucolytic). Twenty patients agreed to participate in a randomized, single-blind clinical trial. All patients underwent endoscopic endonasal ethmoidectomy for nasal polyps. The packing was removed after 48 h and patients were asked to start the same day nasal lavages three times a day. Clinical evaluations were performed: (1) by weighing residual nasal crusts and secretions after 21 +/- 2 days; and (2) by using visual analogue scales to daily record symptom scores. Data are presented as mean +/- SEM. T-test statistics for two independent groups were applied. The mean residual crust and secretion weights were 1,756 +/- 688 mg and 1,033 +/- 422 mg in the pressurized seawater group, 932 +/- 414 mg and 1,222 +/- 435 mg in the antiseptic-mucolytic saline group. No statistical differences were found. Sample size calculations showed that 100 subjects in each group would be necessary to confirm a 700-mg reduction in residual crusts in the antiseptic/mucolytic saline group (power = 0.80; two-sided type-I error = 0.05). Daily symptom score curves were similar in both groups and allowed us to give a description of post-operative complaints. The role of antiseptic, mucolytic and mechanical lavages in preventing post-ethmoidectomy crust formation is discussed.

Anti-obesity and Antidiabetic Effects of Deep Sea Water on ob/ob Mice

Ratones obesos (PDF).

Recently, deep sea water (DSW) has started to receive much attention for therapeutic intervention in some lifestyle diseases. In this study, the anti-obesity and antidiabetic effects of DSW in ob/ob mice were investigated. The animals were randomly divided into two groups with six animals: control group received tap water; the experimental group was treated with DSW of hardness 1000 for 84 days. The body weight gain after 84 days in DSW-fed group was decreased by 7% compared to the control group. The plasma glucose levels in the DSW-fed mice were substantially reduced by 35.4%, as compared to control mice. The results of oral glucose tolerance test revealed that DSW-fed groups significantly increased the glucose disposal after 84 days. DSW increased plasma protein levels of adiponectin and decreased plasma protein levels of resistin, RBP4, and fatty acid binding protein. Moreover, GLUT4 and AMP-activated protein kinase levels in skeletal muscle tissue were increased while peroxisome proliferator-activated receptor ? and adiponectin were decreased in adipose tissue of DSW-fed mice. These results suggest that the antidiabetic and anti-obesity activities of DSW were mediated by modulating the expression of diabetes- and obesity-specific molecules. Taken together, these results provide a possibility that continuous intake of DSW can ameliorate obesity and diabetes.

Efficacy of Isotonic Nasal Wash (Seawater) in the Treatment and Prevention of Rhinitis in Children

Physiomer-CT Lavado nasal 200 (PDF)

Objective: To evaluate the potential of nasal isotonic saline application to prevent reappearance of cold and
flu in children during the winter.
Design: Prospective, multicenter, parallel-group, open, and randomized comparison.
Setting: Eight pediatric outpatient clinics.
Patients: A total of 401 children (aged 6-10 years) with uncomplicated cold or flu.
Interventions:Werandomly assigned patients to 2 treatment groups, one with just standard medication, the other with nasal wash with a modified seawater solution (Physiomer) plus standard medication, and observed them for
12 weeks.
Conclusion: Children in the saline group showed faster resolution of some nasal symptoms during acute illness and less frequent reappearance of rhinitis subsequently.

Physiomer® reduces the chemokine interleukin-;8 production by activated human respiratory epithelial cells

Physiomer e IL8 (PDF).

The authors have recently shown that the transcription factor nuclear factor-B (NF-B) is a central mediator in the NaCl-mediated interleukin (IL)-;8 production by human airway epithelial cells. In this study, it was investigated whether Physiomer®, an isotonic sea water-derived solution commercialized for cleaning the nasal mucosa, impaired the chemokine IL-;8 expression and secretion by human respiratory epithelial cells compared with that obtained with an isotonic 9% NaCl solution.
Primary human bronchial gland (HBG) epithelial cells were incubated either in Physiomer® or in a NaCl 9% solution and activated either with 20 ng·mL–1 tumour necrosis factor-;, or IL-;1ß, respectively. Physiomer® significantly reduced the IL-;8 protein release in basal and activated HBGcells in comparison with that obtained with the 9% NaCl solution. In contrast to the effects of Physiomer® observed on restingHBG cells, Physiomer® did not significantly reduce the level of phosphorylation of the NF-B inhibitor protein IB or the steady-state IL-;8 messenger ribonucleic acid levels in activated HBG cells, suggesting that Physiomer® would have a post-transcriptional effect on IL-;8 expression in activated HBG cells. The authors conclude that Physiomer® is potentially useful in the reduction of airway mucosal inflammation.

Microbial diversity in the deep sea and the underexplored ‘‘rare biosphere’’

Microbios (PDF).

The evolution of marine microbes over billions of years predicts that the composition of microbial communities should be much greater than the published estimates of a few thousand distinct kinds of microbes per liter of seawater. By adopting a massively parallel tag sequencing strategy, we show that bacterial communities of deep water masses of the North Atlantic and diffuse flow hydrothermal vents are one to two orders of magnitude more complex than previously reported for any microbial environment. A relatively small number of different populations dominate all samples, but thousands of low-abundance populations account for most of the observed phylogenetic diversity. This ‘‘rare biosphere’’
is very ancient and may represent a nearly inexhaustible source of genomic innovation. Members of the rare biosphere are highly divergent from each other and, at different times in earth’s history, may have had a profound impact on shaping planetary processes.

Metabolic effects in rats drinking increasing concentrations of sea-water

Metabolic effects in rats drinking (PDF).

1. Research on laboratory rats confirmed that drinking sea-water when dehydrated, was not beneficial and caused impaired renal function.
2. When the concentration of sea-water in the drinking water is gradually increased there is a gradual increase in water uptake and corresponding urine excretion.
3. At 50% sea-water the maximum uptake and excretion is reached. Following this there is a decline in appetite, water uptake and urine secretion.
4. When on 100% sea-water, the creatinine clearances were greater than on tap water, while urine/plasma osmolalities (U/P) averaged 7. The only higher U /P was found in animals drinking sea-water when dehydrated, i.e. a U/P of 11.
5. The urea metabolism appears to be suited to either the need to conserve body water, up to 50% sea-water, or to guarantee an adequate urine production, from 50% sea-water to pure sea-water.
6. It is suggested that when a man is stranded at sea it is not advisable to drink all the fresh water and then be compelled to drink sea-water when dehydrated.
7. It is better to slowly increase the sea-water uptake. This will prolong the time before sea-water needs to be drunk and result in only minor metabolic changes. Return to fresh water will be followed by an immediate return to normal homeostasis.

Pharmacological Activity of Deep-Sea Water: Examination of Hyperlipemia Prevention and Medical Treatment Effect

Lípidos (PDF)

When normal rabbits were administered various samples of deep-sea water, their biochemical values changed within normal limits, and no differences from distilled water administration (control) group levels were observed. Furthermore, no histopathological changes were observed in internal organs on the 28th day after administration.
The serum total cholesterol (T-Cho) and low density lipoprotein cholesterol (LDL-Cho) levels of normal rabbits fed with a 1% cholesterol-containing diet simultaneously administered deep-sea water (desalinated water, hardness 28, 300, and 1200) increased with time up to about 1500 mg/dl. However, the degrees of increase were smaller than those of the control group, which received distilled water. Furthermore, when prepared hyperlipemia rabbits were administered deep-sea water (desalinated water, hardness 28, 300, and 1200), there were no significant changes in aspartate aminotransferase (AST), alanine aminotransferase (ALT), high density lipoprotein cholesterol (HDL-Cho), or triglyceride (TG) levels. On the other hand, T-Cho and LDL-Cho levels were reduced when the rabbits were changed to normal food, and the degree of reduction was more than that of the control group. In the liver and main artery bow, as the hardness of the deep-sea water increased, the accumulation of lipid and permeation of macrophages was reduced. This result was well in agreement with the results of the T-Cho and LDL-Cho levels. From these results, it is clear that deep-sea water controls the increase of serum lipid values (T-Cho and LDL-Cho) of cholesterol-fed rabbits, and promotes the reduction of serum lipid hyperlipemia rabbits. The minerals in deep-sea water greatly influence this effect.

The Influence of Diluted Seawater and Ripening Stage on the Content of Antioxidants in Fruits of Different Tomato Genotypes

Agua de mar y Tomate (PDF).

The aim of this study was to investigate if the combined effect of diluted seawater and ripening can improve the beneficial nutritional properties of tomato fruits from an antioxidant point of view. To reach the goal, different tomato cultivars and breeding lines, genetically modified for ripening, were investigated, and analysis of NADPH and NADP+ as well as of the main antioxidants such as ascorbic acid, lipoic acid, and tocopherols was performed at two ripening stages. The research was conducted on berries of the following genotypes of tomato: cv. Jama, Gimar wild type, Gimar gf, and Gimar nor. The mutant gf is a typical “stay green” mutant, characterized by an incomplete loss of chlorophyll; the nor mutation is characterized by a reduced biosynthesis of ethylene and carotenoids. Both ripening and salinity induced an oxidative stress, and the sensitivity to salt treatment was genotype-dependent. The genotypes cv. Jama and Gimar gf line showed increases in ascorbic acid, lipoic acid, and R-tocopherol during both ripening and salt treatment whereas total ascorbate and tocopherols decreased in the berries from salt-treated plants of Gimar wild type. Ripening also determined decreases in ascorbate and tocopherol amounts in the Gimar nor line where a positive effect of ripening and salinity was observed.

Severe Hypernatremia From Sea Water Ingestion During Near-Drowning in a Hurricane

Hipernatremia por ingesta de agua de mar (PDF).

Ingestion of seawater, whether voluntaryl or accidental, and its clinical sequelae have not been amply reported in the literature. Although seawater ingestion has been directly observed in animal drowning experiments and is believed to occur during human drowning, it has not been reported to play a significant role in producing serum electrolyte abnormalities in neardrowning victims. Because of the high concentration of sodium in seawater (approximately 350 to 500 mmol per liter), ingestion of this fluid and subsequent absorption of electrolytes can lead to hypernatremia. This condition is caused not only by the addition to the body of proportionately more sodium than water, but by water loss from solute diuresis or osmotic diarrhea.
The near-drowning patient whose case is reported in this paper experienced unusual circumstances as the result of a shipwreck during the height of a hurricane; the patient’s immersion and involuntary seawater ingestion led to the development of severe hypematremia. This case is interesting not only because of the patient’s harrowing ordeal, but also because it illustrates the physiologic changes and clinical symptoms that can occur in a victim of near-drowning when significant quantities of seawater are ingested rather than aspirated. To my knowledge, the patient’s serum sodium levels are the highest reported in the literature on near-drowning.

Chemical Speciation of Trace Metals in Seawater: a Review

Elementos traza (PDF).

The recent development of the chemical speciation of trace metals in seawater is described. The speciation studies reveal that metal ion complexation is one of the most important processes in seawater; especially, most bioactive trace metals, such as Fe(III) and Cu, exist as complexes with ligands in dissolved organic matter. The organic ligands in seawater are characterized with metal ions selected by the HSAB concept. A strong organic ligand, which originates from marine microorganisms, is classified as a hard base including carboxylates. The free organic ligand concentrations in seawater are buffered by complexation with excess amounts of Ca and Mg in seawater. The chemical equilibrium model suggested that the concentrations of bioactive free metal ions are at an optimal level to activities of marine microorganisms. For chemical speciation, it is important to have a better understanding of the ecological roles of trace metals in seawater.

Detection, characterization and dynamics of dissolved organic ligands in oceanic waters

Elementos complejos orgánicos (pdf)

Dissolved organic matter in oceanic waters was investigated in terms ofthe interaction with copper. Three classes of organic ligands, namely, L1, L2 and LN that were concentrated by repeated rounds oflyophilization and dialysis, were distinguished from each other by differences in their complexing abilities for copper. Ligands L1 and L2 appeared to be assigned to the group ofweak ligands in the literature. The conditional stability constant of ligand LN for Cu(II) was extremely high and was comparable to that ofEDTA (ethylenediaminetetraacetic acid).
Two types of ligands that were similar to weak ligands L1 and L2, were extracted directly from seawater using immobilized metal ion affinity chromatography (IMAC). lMAC gave a new insight in that the weak ligands were a mixture of at least two types of different organo-chemicalligands and that their dynamics might be active in the water column.

Deep-Sea Water Improves Cardiovascular Hemodynamics in Kurosawa and Kusanagi-Hypercholesterolemic (KHC) Rabbits

Conejos cardiovascular (PDF).

Deep-sea water is rich in minerals, e.g., Mg, Ca, and K which have been considered to be associated with prevention of cardiovascular disease. We investigated the effect of deep-sea water on cardiovascular hemodynamics in Kurosawa and Kusanagi-Hypercholesterolemic (KHC) rabbits. Deep-sea water was pumped in the offing of Cape Muroto in Japan and the mineral constituents were refined to a degree of hardness of 1000. Twenty four 4-month-old KHC rabbits were given refined deep-sea water (n=12) and tap water (n=12) for 6 months. Pressure and flow waves at the ascending aorta were recorded under pentobarbital anesthesia. Systolic, diastolic, pulse and mean arterial pressures and total peripheral resistance were significantly lower in the deep-sea water group than in the control group. There were no significant differences in changes in serum lipid levels, plasma renin and angiotensin converting enzyme activities and electrolyte levels except for Mg2+ after the feeding of the water between the two groups. A slight increase in serum Mg2+ level in the deep-sea water group may not account for the inhibition of mild hypertension. From our results, we conclude that deep-sea water could improve cardiovascular hemodynamics, even though the factors which affect the blood pressure are still unknown.

Effect of drinking saline water and feed shortage on adaptive responses of sheep and camels

Camellos (PDF).

This study investigated the effect of saline load and inadequate feed intake on some of the adaptive physiological responses in female sheep and camels, raised under semi-arid conditions. The experiment comprised five consecutive periods, P1–P5, of 40 days each, during which levels of both energy and protein were gradually decreased by increasing the roughage portion in the diet. Sheep and camels were divided into three groups according to the type of drinking water; namely a fresh water (F) group (280 parts per million total dissolved salts; ppm TDS), low saline (LS) group (7650 ppm TDS) and high saline (HS) group (13,535 ppm TDS). Saline water was obtained by diluting seawater with tap water.
In sheep, live body weights (BWs) decreased significantly (p < 0.01) with decreasing nutrient intake, with average final loss equal to 8.4%. Plasma glucose decreased with decreasing protein intake, but as energy intake increased the effect of protein shortage disappeared. Also plasma glucose levels in sheep decreased from a level of 3.51 mmol/l in the F group to 2.89 mmol/l in the HS group. Concentrations of liver enzymes aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT) in sheep increased in plasma in relation to saline load especially at low nutritional level. The activity of the acetylcholine esterase enzyme (AChE) in its three sites; blood, red blood cells and plasma was depressed significantly by both saline load and decreased feed intake. At the P2 period, salinity depressed acetylcholine in its three sites to 60–67% in the HS group as compared to the control. The depression during the P5 period reached 41–54%. Extracellular fluids (ECFs), interstitial fluids (ISF), plasma volume (PV) and blood volume (BV) in the ewes decreased (p < 0.05) by increasing salinity concentration. Decreasing feed intake lowered ECF, ISF and BV from the P2 period.
In camels, live BWs decreased insignificantly by decreasing feed intake with a final BW loss of 1.9%. Plasma glucose was not affected by salinity. Protein deficiencies had no effect on plasma AST of camels, but both salinity and low level of nutrient intake affected the concentration of enzyme ALT. Nutrient shortages and saline load affected activity of AChE at the P4 and P5 periods. The inhibition of the enzyme activity during P5 due to high salinity treatment reached 91% in blood, 63% in RBCs and 50% in plasma as compared to the control group. Body fluid compartments of camels were not affected by salinity, only by reduced feed intake. The results indicated better tolerance of camels than sheep to both saline load and feed shortage.
© 2002 Published by Elsevier Science B.V.

Deep-Sea Water Suction Techno

Bombeo de deep seawater (PDF).

In the area of deep-sea water suction technology, Furukawa Electric has built an excellent  track record of laying eleven deep-sea water suction pipes in seven locations since its first domestic laying in Muroto City, Kochi Prefecture in 1988. During this time, we have introduced various improvements in the laying method as well as in the design of material structures including suction pipes, thereby making it possible to manufacture and install the suction pipes up to 280 mm in diameter, 680 m in laying depth, and 7200 m in pipe length

Effects of Hot Deep Seawater Bathing on the Immune Cell Distribution in Peripheral Blood from Healthy Young Men

Baño de agua de mar caliente y sistema inmune (pdf)

Objectives: Deep seawater (DSW) utilization technology has been developed for the fields of medicine and health, among others. To clarify the health effects of DSW as compared with surface seawater (SSW) or tap water (TW), we investigated the changes of immune cell distribution of the peripheral blood, or subjective judgment scores, after hot water bathing.
Methods: Ten healthy young men were immersed for 10 min in DSW, SSW and TW heated to 42°C. Blood samples were collected before bathing, immediately after bathing and 60 min after bathing. Total and differential numbers of leucocytes and lymphocyte subsets (CD3, CD4, CD8, CD19, CD16, and CD56) were examined using an automated hematology analyzer and a flow cytometer, respectively. The subjective judgment scores were obtained by an oral comprehension test.
Results: Since the pre-bathing leukocyte count in the TW group was significantly different from those in the DSW and SSW groups, we excluded the findings of TW bathing from consideration. In hot DSW bathing, CD8-lymphocytes increased significantly immediately after bathing (p<0.05), in contrast to hot SSW bathing, in which no significant changes were detected in the lymphocyte subsets. Additionally, there were no significant changes between repeated measurements in the subjective judgment scores, though the score of thermal sensation in SSW bathing showed a significantly higher value immediately after bathing than before bathing (p<0.01).
Conclusions: Our findings suggest that increased CD8-lymphocytes in hot DSW bathing may improve human immune function as well as hot springs do, as compared with SSW bathing. Although hot DSW bathing may have the ability to change human immune cell distribution, well-designed studies are needed to clarify the health effects including not only DSW and SSW but also TW.

Difference between Deep Seawater and Surface Seawater in the Preventive Effect of Atherosclerosis

Aterosclerosis (PDF).

Using surface and deep seawater collected in the sea area of Muroto Cape (Kochi, Japan), desalinated drinking samples of about 1200 hardness were prepared and examined for the effects on the prevention of atherosclerosis in dietary induced hyperlipidemia rabbits. The plasma LDL cholesterol level was lower in the deep seawater group than in the surface seawater group. GPx activity was significantly higher in the deep seawater group than in the control group, while there was no difference between the surface seawater and control groups. The level of LPO was also significantly lower in the deep seawater group than in the control group. The Sudan IV lipid stained area ratio on the inner surface of the aorta was significantly lower in the deep seawater groups than in the control group, while there was no difference between the surface seawater and control groups. The oil red O stained cross section of the aorta in the control and surface seawater administration group foam cells had accumulated to form thick layers, while in the deep seawater administration group, the degree of their accumulation was very low. These results suggested that the deep seawater was useful for the prevention of hyperlipidemia and arteriosclerosis compared to the surface seawater, and it was found that reduction of the LDL cholesterol level and enhancement of GPx activity were involved in its effects.

Reduction of Allergic Skin Responses and Serum Allergen-Specific IgE and IgE-Inducing Cytokines by Drinking Deep-Sea Water in Patients with Allergic Rhinitis

Agua de mar y renitis alérgica (pdf)

Deep-sea water intake reduces allergic skin responses and serum levels of total IgE, Japanese cedar pollen-specific IgE, interleukin (IL)-4, IL-6, IL-13, and IL-18 in patients with allergic rhinitis, while distilled water intake fails to do so.

The manzanar project: Towards a solution to poverty, hunger, environmental pollution, and global warming through sea water aquaculture and silviculture in deserts

Agricultura (PDF).

Dr. Gordon Sato is a former Editor-in-Chief of In Vitro Cellular and Developmental Biology, President of the Tissue Culture Association (now Society for In Vitro Biology), and Director of the W. Alton Jones Cell Science Center (now Adirondack Biomedical Center). He began pilot experiments on the Manzanar Project at test sites in the Salton Sea while a Professor of Biology at the University of California, San Diego and continued the project in the laboratory at the Cell Center in Lake Placid, NY and at Eritrean test sites during their war of independence. Since 1994, he spends up to 10 mo. per yr in Eritrea where he directs the Manzanar Project and trains young Eritrean scientists in the field in the area of what he refers to as «low-tech biotech.» The name of the Manzanar Project was inspired by the camp in California where Dr. Sato and his family were interned during World War II.